How do I use a microscope correctly ? To be able to use a microscope to properly consider the following steps :
a. Finding
the visual field by adjusting irradiation to produce the visual field
is the mengatu mirror while looking at that light into the eyepiece to
the diaphragm , resulting in optimum reflectivity . The bright part is called the spherical field of view .
b
Adjust the focus of the microscope with a magnification weak or shadows
on the table Put preparations preparations , clamped with tongs as he
watched from the side of the tube microscope microscope lowered with a
rough player , do careful not to touch the objective lens preparations .
Then look through the eyepiece and slowly microscope Bring up the tube so that the object is clearly visible. Once the object looks , smooth player to rotate forward or backward to get the best possible image . Magnification
of the microscope is obtained by multiplying the numbers in the
objective lens with the numbers listed in the ocular lens . For example, the objective lens 5x 10x 50x eyepiece then perbesarannya .
c . Adjusting
the focus of the microscope ( shadow with powerful magnification ) To
obtain the shadow , can be done by changing the objective lens has a
magnification weaker by the stronger . For
example, an objective lens with a magnification of 5x to 10x or 40x
replaced by rotating the revolver until you hear terdetak . Smooth player rotated forward or backward in order to obtain a clearer object .
d . Set the microscope to position stored Upon completion microscope is
used , adjust the microscope with position ready to be stored in the
following way :
1 ) Tube microscope is raised .
2 ) Mixture taken .
3 ) The objective lens is the weakest lowered as low as played exactly the microscope until the hole table .
4 ) The diaphragm is closed again .
5 ) Condenser lowered and mirrors in an upright position .
6 ) Raise the microscope with the right hand carefully holding the arm
and prop feet mikrokop microscope with the left hand and then put into
place and locked .
How to make preparations :
1 ) Make preparations without incision : To make a wet mount without an incision , for example on
time observation of microorganisms present in water . How: water to be observed , taken with a Pasteur pipette and placed on
glass objective and cover with a glass cover , observe with a
microscope .
2
) Make an incision preparations : Make preparations in organ organism ,
for example cross-section of leaves , stems , roots , muscles , etc.
How: slashing organ as thin as possible , to make a good and thin
incision with an instrument called a microtome , but if not using a microtome can have razor sharp .
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